Reporter
GG96
Part:BBa_K2145001:Experience
Designed by: Melody Wu, Laura Xinyu Chen, Katie Maruna Group: iGEM16_Alverno_CA (2016-10-14)
Applications of BBa_K2145001
Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
User Reviews
UNIQ79f52942f23d58c1-partinfo-00000000-QINU UNIQ79f52942f23d58c1-partinfo-00000001-QINU