Generator

Part:BBa_I739001

Designed by: Christian Kemmer, Stefan Luzi   Group: iGEM07_ETHZ   (2007-09-27)
Revision as of 11:41, 11 October 2007 by Stefan Luzi (Talk | contribs)

Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)

Part Structure

The Biobrick encodes LVA-tagged tetR (BBa_C0040) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.

Mode of Action

TetR binds to the pTet regulator (BBa_R0040) and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.


Testing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None