Composite

Part:BBa_K1962012

Designed by: Frank Sargent   Group: iGEM16_Dundee   (2016-10-12)
Revision as of 18:54, 22 October 2016 by Frulhuq (Talk | contribs)


A device for expression of Im-E3 in response to bile salts

This is a composite part that is designed to induce production of the Immunity Protein to Colicin E3 in the presence of bile. The composite part consists of the bile salt responsive promoter (BBa_K318514), RBS (BBa_B0030) and the Colicin E3 Immunity protein (BBa_K1962004). For best results, use in conjunction with the transcriptional activator RamA (BBa_K1962009).


Usage and Biology

T--Dundee--Results17.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 471
    Illegal XhoI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None