Part:BBa_K530002

CRTI

This part has been improved by the DTU-Denmark 2016 team by removing the illegal restriction site BgIII at bp 204, making the part compatible with the RFC21 standard. Please see BBa_K2117013 for details of the new and improved part.

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Phytoene Desaturase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes the conversion of Phytoene to Lycopene.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 204
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1108
  Illegal SapI site found at 508
  

Characterization of performance

Below we can see the beta-carotene production levels on a per cell basis for different combinations of parts involved in beta-carotene synthesis. These are compared with a wild type control. tHMG1 is used to funnel more initial substrate into the pathway and although useful, was not submitted as a part. 2I indicates that two copies of crtI were used.

Plasmid Map

Sequencing

This is the sequencing for colony 7.