Plasmid

Part:BBa_J61148

Designed by: John Anderson   Group: Arkin Lab   (2007-09-04)
Revision as of 02:30, 5 September 2007 by JCAnderson (Talk | contribs)

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pBca9145 basic pUC/Amp plasmid in BBb

pBca9145 is essentially the pSB1A2 of the BBb format. It contains an EcoRI-BglII-Part-BamHI-XhoI in the EcoRI/PstI region of pSB1A2.

The annotated version of this plasmid is here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 2058
    Illegal suffix found in sequence at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2058
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1097


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