Composite

Part:BBa_K2066038:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)
Revision as of 03:12, 20 October 2016 by KalenClifton (Talk | contribs) (→‎Design Notes)


Sigma54 Enhancer 57S, no DT, UNS standard


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 111
    Illegal NheI site found at 206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 151
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed for Gibson Assembly from the 57S plasmid from Amit et al. 2011 “Building Synthetic Enhancers from the Ground Up” paper, with a UNS backbone to create K2066038. Note that this part has no double terminator, as the original 57S plasmid did not have one after the mCherry + NRII coding regions.

Source

to be completed

References