Measurement
Part:BBa_K2066059:Design
Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss Group: iGEM16_William_and_Mary (2016-10-12)
Revision as of 00:59, 20 October 2016 by KalenClifton (Talk | contribs)
No part name specified with partinfo tag.
No part name specified with partinfo tag.
Design Notes
This part was designed to perform RiboJ characterization verifying and building on that performed in Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”).
Source
This part was created using Gibson Assembly methods. We used K2066035 as a template and an amplicon containing the relevant promoter sequence +/- RiboJ combination as the insert. RiboJ sequence sfGFP sequence from Lou et al., Supplement section V. sfGFP sequence derived and modified from Lou et al., Supplement section V. The UNS sequences are from Torella et al. 2013.