Composite

Part:BBa_K2066014:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-08-31)
Revision as of 00:33, 20 October 2016 by KalenClifton (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


plLacO-1+RiboJ+sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 224


Design Notes

Part designed to be compared with K2066026 (without RiboJ) to see the effect of RiboJ on the transfer function.

Source

This part was created using IDT DNA Synthesis.

The UNS2 and UNS3 sequences are taken from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”). RiboJ sequence is from Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context").

References