Regulatory

Part:BBa_K1886017:Design

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-12)
Revision as of 18:06, 17 October 2016 by Lejian (Talk | contribs) (→‎Source)


broken_Ptet


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence for pTet inverting regulator. Promoter is constitutively ON and repressed by TetR. TetR repression is inhibited by the addition of tetracycline or its analog, aTc.

Source

This promoter is different from BBa_R0040. R0040 has repeated sequences of tet operator, while K1886017 has only one tet operator.This promoter is bought in Addgene company.

References