Reporter
GG98

Part:BBa_K2145105

Designed by: Laura Xinyu Chen, Katie Maruna, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)
Revision as of 20:05, 16 October 2016 by Mwu (Talk | contribs)


GFP/RFP reporter (Reverse/Reverse) with random 500bp spacer

This part contains two fluorescent protein coding sites (for RFP and GFP) and was assembled using Golden Gate Assembly. To make plasmid, parts were taken from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled to make new part with a 500bp spacer in between.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1661
    Illegal AgeI site found at 1773
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None