Part:BBa_K2003011

Inducible Green Fluorescent Protein UnaG+6xHis-tag+Flexible linker
As part of this iGEM project, research has been done on the newly discovered green fluorescent protein called UnaG. This protein is interesting foremost because it is about half the size of other fluorescent proteins that are being used today, making it suitable as a fusion protein. In addition it fluoresces only when a bilirubin molecule is bound non-covalently to the protein. This feature could make UnaG suitable as an inducible marker by the addition and removal of bilirubin to cells. Moreover, unlike all other GFP derivatives used today, UnaG does not need molecular oxygen in order to fluoresce, meaning that it can be used as a marker protein in research under hypoxic conditions. These cool features are among the reasons that motivated us to add this protein to the iGEM toolbox. During the summer we created three different UnaG BioBricks: one with a hexahistidine tag suitable for affinity chromatography (BBa_K2003010), one biobrick with only the flexible linker attached to UnaG suitable for fusing to other proteins (BBa_K2003012), and a third with the hexahistidine tag and a flexible linker of six amino acids (BBa_K2003011).
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]