Protein_Domain

Part:BBa_K1951009

Designed by: claire raynaud   Group: iGEM16_Aix-Marseille   (2016-10-13)
Revision as of 21:36, 15 October 2016 by Loulou (Talk | contribs) (→‎Metal Biosorption Capacity)


FliC Desulfovibrio producer

FliC, the main swimming protein (K1951006 + K880005)

General

Flagellin C (FliC) protein from Desulfovibrio vulgaris strain is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains as the capacity of swimming given by the flagellum confers a great selective advantage.

Metal Biosorption Capacity

It has been demonstrated that Flagellin has the ability to adsorb precious metal on its surface such as platinum, palladium gold [1]...

We assess the production of the protein by a SDS page/comassie test. We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 461


  1. ↑ Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract.
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