Coding

Part:BBa_K2065002

Designed by: Tom van Sonsbeek   Group: iGEM16_TU-Eindhoven   (2016-10-02)
Revision as of 21:35, 15 October 2016 by Bram (Talk | contribs)

T14-3-3 (S71L) - T14-3-3


Usage and Biology


Sequence

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 1389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 1389
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 1389
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 1389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1234
    Illegal EcoRI site found at 1389
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 937

Bioluminescence confirmation

LargeBiT-GGS10-CT52 Statistics

Protein Specifications
General Information Number of amino acids 241
Molecular weight 25770.01
Theoretical pi 5.14
Extinction coefficient 21430
Formula C1140H1807N313O359S4
Total numbers of atons 3623
Amino Acid Composition Amino Acid Frequency Percentage(%)
Ala(A) 11 4.6
Arg(R 9 3.7
Asn(N) 11 4.6
Asp(D) 12 5.0
Cys(C) 0 0
Gln(Q) 11 4.6
Glu(E) 16 6.6
Gly(G) 35 14.5
His(H) 5 2.1
Ile(I) 19 7.9
Leu(L) 20 8.3
Lys(K) 11 4.6
Met(M) 4 1.7
Phe(F) 8 3.3
Pro(P) 7 2.9
Ser(S) 19 7.9
Thr(T) 13 5.4
Trp(W) 2 0.8
Tyr(T) 7 2.9
Val(V) 21 8.7
Pyl(O) 0 0.0
Sec(U) 0 0.0



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Categories
Parameters
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