Part:BBa_K1993001
CCR7
Chemokine receptors are found on the surface of certain cells that interact with chemokines. They have a 7-transmembrane (7TM) structure and couples to G-protein for signal transduction within a cell[1] (Figure 1). Following interaction with their specific chemokine ligands, chemokine receptors trigger a flux inintracellular calcium (Ca2+) ions which initiate the onset of chemotaxis that traffics the cell to a desired location (Figure 2).
Figure 1. typical structure of a chemokine receptor.
References
[1]Allen, Samantha J.; Crown, Susan E.; Handel, Tracy M. (2007-01-01). "Chemokine: receptor structure, interactions, and antagonism". Annual Review of Immunology. 25: 787–820.
[2] Griffith J W, Sokol C L, Luster A D. Chemokines and chemokine receptors: positioning cells for host defense and immunity.[J]. Annual Review of Immunology, 2014, 32(1):659-702.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1111
Illegal SapI site found at 1017
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Design
Background
Inflammmation During the inlflammation process of multiple diseases,such as IBD and artritis,chemoattraction plays a pivotal role,taking the advantae of chemokines and cytokine in target to its correspongding receptors.
Chemotaxis Therefore,stem-cell researchers attempt to transport MSC, a kind of mesenchymal stem cell with outstanding capability of inducing issue regenereation and anti-infllammation,to the injured position for cell therapy.Genetically engineered MSCs with upregulated chemokine receptor can obtain more efficient chemoatrraction ability.
Subparts
Promotor
Chemokine receptors are expressed under the control of constructive promoter EF-1α therefore they could be consistently expressed by MSCs.EF1-α induces the prolonged transcription from plasimid DNA to RNA in Human MSCs,and then the RNA will be translated into proteins and expresses in MSCs, like chemokine receptors are integrated onto the celluar membrane.
Chemokine Receptor
Chemokine receptors are cytokine receptors found on the surface of certain cells that interact with a type of cytokine called a chemokine. They have a 7-transmembrane (7TM) structure and couples to G-protein for signal transduction within a cell [^emphasize] (CXCR5)(Figure 1). Following interaction with their specific chemokine ligands, chemokine receptors trigger a flux inintracellular calcium (Ca2+) ions which initiate the onset of chemotaxis that traffics the cell to a desired location (Figure 2).
CXCR4
disease
function
CXCR5
disease
function
Marking protein
- eFTH
- eGFP
- Luciferase
- dTomato
IRES
T2A
attB1/attB2
- attB1
- attB2
Composite parts
Protein coding sequences
- CCR7
- CXCR1
- CXCR4
- CCR5
Composite parts
Luciferase-IRES-eGFP
Luciferase-T2A-dtomato-T2A-hFTH
CXCR4-IRES-eGFP
CXCR5-IRES-eGFP
CXCR4-T2A-Luciferase-IRES-eGFP
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