DNA

Part:BBa_K1951005

Designed by: claire raynaud   Group: iGEM16_Aix-Marseille   (2016-10-10)
Revision as of 13:09, 15 October 2016 by Clacla02 (Talk | contribs)


FliC E.coli coding sequence

This coding sequence codes the Flagellin C (FliC) protein, filament of the flagellum from Escherichia coli strain.

T--Aix-Marseille--flagellum.jpeg

FliC is the main protein constitutive of the flagellum filament and is involved to promote bacterial swimming. In the possession of flagellum, bacteria can move what confers them a selective advantage.

It has been demonstrated that Flagellin has the ability to adsorbe precious metal such as Platinum, gold..

Design resum

Codons have been optimized for Escherichia coli allowing a high transcription level.

Every forbidden restriction sites have been removed and sequence is optimital.

Proof of fonctionnality

This sequence has been tested under the control of a strong promoter and validated as fonctionnal. For further information, go on the following link : BBa_K1951008 design


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None