Regulatory

Part:BBa_K2165004:Design

Designed by: Roya Amini-Naieni   Group: iGEM16_Washington   (2016-10-14)
Revision as of 03:03, 15 October 2016 by Roygoli (Talk | contribs) (→‎Design Notes)


CUP1 yeast inducible promoter with RFC[10] restriction sites removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). Base pair number 106 was originally a guanine and was substituted with an adenine in the 5' to 3' direction to eliminate the XbaI site in the middle of the sequence.

Source

The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae.

References