Coding

Part:BBa_K1981004:Design

Designed by: Yulei Dang, Xinhao Song   Group: iGEM16_NKU_China   (2016-10-14)
Revision as of 17:21, 14 October 2016 by Songxinhao (Talk | contribs) (→‎Source)

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lsrD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Point mutation was performed to mutate the restriction site of EcoRI at 52 bp and SpeI 345 bp. Condon optimization was performed to meet the manufacture requirement of IDT to synthesize the DNA fragment.


Source

Sequence was acquired from genomic of Bacillus thuringiensis serovar thuringiensis str. IS5056. Part was chemically synthesized.

References