Coding
eGFP

Part:BBa_K2075001

Designed by: Kim Luehmann   Group: iGEM16_Hannover   (2016-10-13)
Revision as of 15:17, 14 October 2016 by Kiml (Talk | contribs)

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enhanced GFP

The eGFP is a part which synthesize a green fluorescence protein (GFP). Exposed under blue or UV light it emits a bright green light. The enhanced GFP (eGFP) has a higher intensity emission than the wildtyp GFP. The origin of the GFP is the jellyfish Aequorea victoria. Today the GFP is an essential fluorescence reporter protein for biologie especially cellbiologie. It can be connect to other proteins to detect the expression of these proteins and their location. We used the eGFP to detect our TAL effectors on a chip, spotted with oligonucleotides. these oligonucleotides includes the target sequenz of the TALE Hax3 – 2xNN (A C A C C C G/A G/A N C A). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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