Part:BBa_K1919304
livK
LivK is the periplasmic binding protein of the LS or leucine specific ABC transport system in E. coli K-12.LivK is synthesized as a precursor; a 23 amino acid signal sequence is cleaved upon export to the periplasm.The protein consists of two domains connected by a 3-stranded hinge; in the closed form, ligands (phenylalanine and leucine) are bound in a cleft formed between the two domains; binding is stabilized by hydrogen bonds and van der Waals contacts. This device constructed in our team contains a promoter J23100, a RBS, livK coding region and a terminator. The device can be transferred into E.coli and overexpress livK directly. The expression of the livK RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livK has been highly expressed in E.coli BL21 and E.coli DH5a.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 895
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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