Reporter
mCherry
Part:BBa_J06504
Designed by: ytwang Group: iGEM2005 (2005-07-18)
monomeric RFP optimized for bacteria
mRFP1-derived, altered to be a BioBrick by removing a PstI site and adding BioBrick ends. [mRFP1 was itself a derived from DsRed (via 33 mutations!)]
mCherry is one of several "second-generation" monomeric fluorescent proteins developed in Roger Tsien's laboratory at UCSD (cf., Nature Biotechnology 22, 1567 - 1572 (2004). The emission wavelengths are 584 and 607nm [http://www.pnas.org/cgi/content/abstract/99/12/7877].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/reporter/rfp
//function/reporter/fluorescence
//function/reporter/fluorescence
abs | -- |
biology | |
emission | 610 |
emit | 610 |
excitation | 587 |
excite | 587 |
lum | -- |
protein | mCherry2 |
tag | None |