Composite

Part:BBa_K2139013

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)
Revision as of 06:23, 14 October 2016 by Registry (Talk | contribs)

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Ptac with g12

Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exoglucanases for the direct conversion of cellulose into monomeric glucose. The polymerization of the protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX. This construct was synthesized for use in Caulobacter Cresentus and is codon optimized to contain a high GC content.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 864


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Parameters
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