Reporter

Part:BBa_K1997014

Designed by: Xinyuan Qiu   Group: iGEM16_NUDT_CHINA   (2016-10-13)
Revision as of 11:05, 13 October 2016 by Registry (Talk | contribs)

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GFP10->Zif268->GFP11->RBS->GFP1-9

This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 208
    Illegal AgeI site found at 292
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 380
    Illegal BsaI.rc site found at 112
    Illegal BsaI.rc site found at 443


[edit]
Categories
//awards/part_collection/2016
Parameters
None