Protein_Domain
Intein

Part:BBa_K1947002

Designed by: zhang xucheng   Group: iGEM16_NEFU_China   (2016-10-13)
Revision as of 09:27, 13 October 2016 by Registry (Talk | contribs)

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Intein

This sequence encodes a short polypeptide which can be specifically recognized and spliced by DL-Dithiothreitol（DTT）. In our case we add this short sequence between the recombinant protein which we want to purify and the linker protein Spytag, in order to separate the recombinant protein form the Magnetosome.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 517
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 437


[edit]
Categories
//proteindomain/cleavage
Parameters
None