Coding
MymTsfGFP

Part:BBa_K1980003:Design

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 20:45, 12 October 2016 by Sgarforth (Talk | contribs)


MymT sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 595
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This protein has a C terminal hexahistidine tag for purification and has been codon optimised for E. coli.


Source

MymT is originally from Mycobacterium tuberculosis. We ordered it as codon optimised DNA from IDT.

References