Coding

Part:BBa_K1887000

Designed by: Yifei Lu   Group: iGEM16_TMMU_China   (2016-09-27)
Revision as of 04:00, 12 October 2016 by Huqiwen (Talk | contribs)


LacZ The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Usage and Biology

References

Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.


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