Composite

Part:BBa_K1897007:Design

Designed by: Wong Chi Yan   Group: iGEM16_NUS_Singapore   (2016-10-10)
Revision as of 19:29, 11 October 2016 by Cyr95 (Talk | contribs) (→‎References)


Complete Has operon (controlling expression of luxR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1691
    Illegal NheI site found at 1714
    Illegal NotI site found at 3609
    Illegal NotI site found at 4403
    Illegal NotI site found at 4527
    Illegal NotI site found at 5531
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1720
    Illegal BamHI site found at 4476
    Illegal BamHI site found at 4810
    Illegal BamHI site found at 5466
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1767
    Illegal NgoMIV site found at 1771
    Illegal NgoMIV site found at 1827
    Illegal NgoMIV site found at 1945
    Illegal NgoMIV site found at 2099
    Illegal NgoMIV site found at 2164
    Illegal NgoMIV site found at 2500
    Illegal AgeI site found at 1404
    Illegal AgeI site found at 1516
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NIL


Source

The complete Has operon signalling systme consists of many genes that have been synthesised together. The sequences for the original Has system genes that encode the signalling proteins (HasR, HasS and HasI) were obtained from the Serratia marcescens genome and documented in the National Center for Biotechnology Information (NCBI) database (http://www.ncbi.nlm.nih.gov/nuccore/X81195.2). The Has operon promoter was discovered and discussed by Biville et al. (2004) and we obtained the sequence based on their results.The luxR gene (BBa_C0062), Ribosome Binding Site (BBa_B0034), mRFP (BBa_E1010), terminators (BBa_B0015) and Ampicillin resistance gene (BBa_P1002) are all Biobrick parts and their sequences obtained from the Registry of Standard Biological Parts. There are also

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Cescau, S., Cwerman, H., Letoffe, S., Delepelaire, P., Wandersman, C., & Biville, F. (2007). Heme acquisition by hemophores. Biometals, 20(3-4), 603-613.

Rossi, M. S., Paquelin, A., Ghigo, J. M., & Wandersman, C. (2003). Haemophore‐mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules. Molecular microbiology, 48(6), 1467-1480.

Wandersman, C., & Delepelaire, P. (2004). Bacterial iron sources: from siderophores to hemophores. Annu. Rev. Microbiol., 58, 611-647.