Coding
Csp1sfGFP

Part:BBa_K1980001

Designed by: Sam Garforth   Group: iGEM16_Oxford   (2016-10-11)
Revision as of 13:55, 11 October 2016 by Registry (Talk | contribs)

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TAT Copper Storage Protein 1 sfGFP

Copper Storage protein 1 (Csp1) is a tetrameric copper storage protein found in the periplasm of Methylosinus trichosporium OB3b. We investigated whether this part could act as a copper chelator when expressed in E. coli. We modified the protein by adding a TAT signal peptide from the E. coli enzyme CueO in place of the native TAT sequence and added a c terminal sfGFP fluorescence protein with a C terminal his tag in an attempt to direct the protein to the periplasm. The Csp1 and sfGFP are separated by a short hydrophilic, flexible linker.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 886
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//binding/metal
//cds/reporter/gfp
Parameters
None