Coding

Part:BBa_K1887000:Design

Designed by: Yifei Lu   Group: iGEM16_TMMU_China   (2016-09-27)
Revision as of 03:25, 9 October 2016 by Huqiwen (Talk | contribs) (→‎Source)


LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lacZ gene from Lactobacillus acidophilus was introduced into the His locus of L. Lactis genome by temperature sensitive vector, which makes the knocked-in strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).

Source

Using the genomic DNA of Lactobacillus acidophilus as the template, the lacZ gene product is obtained by PCR and cloned into the pSB1C3 plasmid.

References