Regulatory

Part:BBa_K2151000:Design

Designed by: Matej Vucak, Katy Baker, James Provan   Group: iGEM16_Glasgow   (2016-08-30)
Revision as of 20:55, 8 October 2016 by Mvucak (Talk | contribs) (→‎Source)


pHLBA, strong constitutive S. thermophilus promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was not altered for use in E. coli as the aim was to achieve expression in S. thermophilus.

Source

  • Sequence obtained from 'Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene'.
  • Synthesized by IDT as an oligo-nucleotide

References

Slos, P., Bourquin, J. C., Lemoine, Y. and Mercenier, A. (1991) 'ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL PROMOTERS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS', Applied and Environmental Microbiology, 57(5), pp. 1333-1339.