Regulatory

Part:BBa_K2151000

Designed by: Matej Vucak, Katy Baker, James Provan   Group: iGEM16_Glasgow   (2016-08-30)
Revision as of 18:54, 8 October 2016 by Mvucak (Talk | contribs) (Added long description using main body.)


pHLBA, strong constitutive S. thermophilus promoter


This part consists of pHLBA, a strong constitutive bacterial promoter, contained within pSB1C3. pHLBA was initially identified in studies of Lactobacillus bulgaricus where it drives the expression of histone-like proteins. pHLBA is likely functional in all lactic acid bacteria.

We used pHLBA as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of pHLBA using GFP revealed promoter strength on par with strong Anderson promoters (93% as strong as J23100, 132% as strong as J23101).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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