Coding

Part:BBa_K1969002:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-05)
Revision as of 14:01, 8 October 2016 by StevenLai (Talk | contribs) (→‎Design Notes)


Homo sapiens adrenoceptor beta 2 (ADRB2) with 6 x His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562


Design Notes

We used site-specific mutagenesis (G at 192bp to A) to make it become assembly compatible.

Source

The sequence for Homo sapiens adrenoceptor beta 2 is from human genomic sequence, the sequence for 6 x his tag was added artificially.

References