Device

Part:BBa_K1886016

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-07)
Revision as of 12:45, 7 October 2016 by Registry (Talk | contribs)

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AND GATE-input_ara+IPTG

This is the input part of AND GATE. After being induced by L-arabinose, pbad promoter activates the expression of T7 RNA polymerase which contains two amber mutations (T7 ptag) . After being induced by IPTG, plac promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, only when the two inputs exist together, T7 RNA polymerase can be expressed, and then T7 promoter can be activated to express the downstream gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 171
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 174
    Illegal BsaI.rc site found at 4155


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Categories
Parameters
None