Coding

Part:BBa_K1969001:Design

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-05)
Revision as of 09:02, 5 October 2016 by Registry (Talk | contribs)

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Homo sapiens adrenoceptor beta 2 (ADRB2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562


Design Notes

First, it has no obvious tag for researchers to detect it directly, so we further add a 6xhis tag at its C terminus. Second, as the N terminus sequence may decide the membrane localization in different host cells, we used the C terminus sequence of GPCR RI7 and STE2 which were both reported to be successfully located to yeast membrane to replace the corresponding sequence of ADRB2.


Source

Its from human genomic sequence.

References