Help:IGEM 07 Parts Kit

The iGEM 2007 Parts Kit was shipped to teams this May. The collection of four plates contains all parts available in the Registry as of mid-Spring 2007. Here is what the kit looks like.

Storage

Store the DNA Parts Kit with the plastic lid and the adhesive foil cover in the -20C freezer.

Usage

The DNA Parts Kit is a tool available to you to perform synthetic biology with standard biological parts. It contains dry DNA of ALL of the parts that are available in the Registry up until May 2007. You will transform the DNA into cells and make your own glycerol stocks of any part that you wish. It does not contain enough DNA to do assembly. To use the DNA in the Parts Kit follow the following instructions:

1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick™ part that you want
2. Add 15 uL of diH2O (deionized water)
3. Take 1uL DNA and [http://openwetware.org/wiki/Bacterial_Transformation transform] into your desired competent cells, plate out on a plate with the correct antibiotic* and grow overnight. Your goal here is to obtain single colonies.
4. Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow ~18 hours.
5. Use the resulting culture to miniprep the DNA AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]).

*To know which antibiotics to use, look at the plasmid that the part is in. The naming scheme for plasmids is specifically designed to indicate antibiotic resistance.

Part Location

You can locate a part in two different ways:

Targeted search

If you know which part you are looking for, you can enter the part number in the 'Jump to Part' search box:

From here you will be taken to the part's Main Page. On the left side of the page there will be a pink box. This is the Part navigation box. The link to Physical DNA is where you will find the location of the part.

The locations that appear on the Physical DNA page will correspond to the iGEM 2007 DNA Parts Kit. It will include the plate # (Plates 1-4), the well location (e.g. A12), and the plasmid that the part is in.

Browsing parts

In the DNA Repositories section of the Registry, you will find this page that shows the four plates in the DNA parts kit. By clicking on any of the links, you will see all of the parts in each particular plate.

Plasmid Location

To find the plasmids that are available in the DNA Parts Kit, you need to do a targeted search (see above) for BBa_P1010. BBa_P1010 is a CcdB cell death gene that we use as the requisite part when "storing" each of the BioBrick™ plasmids. When you do the search you will go, as described above, to the Physical DNA page and see which plasmids BBa_P1010 is available in. Follow the steps above to locate, resuspend and transform the DNA.