Generator

Part:BBa_K1741000:Design

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)
Revision as of 23:17, 26 September 2015 by MartaZ (Talk | contribs)

sfGFP under arabinose promoter s1 without AraC (Arashort1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 53
    Illegal SapI.rc site found at 352


Design Notes

We fused the araBAD promoter without AraC reading frame, as other sugar induced promoters to sfGFP.

picture Uampoznanarabinose2xlb.png

Source

All promoter fragments derive from Escherichia coli K12, strain DH5α, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.

References

New shorter versions of arabinose induced promoters, all originating from E. coli genome were compared to the biobrick BBa_K1481002 (https://parts.igem.org/Part:BBa_K1481002), provided last year by Poznan_Bioinf team.