Coding

Part:BBa_K1680009

Designed by: Katharina Sporbeck   Group: iGEM15_Tuebingen   (2015-09-17)
Revision as of 00:25, 26 September 2015 by KatharinaS (Talk | contribs)

NanoLuc Luciferase

This part contains the protein coding region for the NanoLuc(tm) luciferase, which is originally designed by Promega Corporation (http://bit.ly/1Vgmab3, free for research use). We codon-optimized the part for yeast. The NanoLuc is smaller than other luciferases while yielding equal or stronger luminescence readings.


PSUC-conc.png RLU measurement of the NanoLuc under pSUC2 promoter BBa_K950003 control, normalized to OD600.

Team Tuebingen mpr 6h.jpg RLU measurement of the NanoLuc under pFET3 promoter BBa_K950000 control, normalized to OD600.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/reporter
//chassis/eukaryote/yeast
//function/reporter/light
Parameters
None