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Part:BBa_K1806005:Experience

Designed by: İBRAHİM YASİR ORHAN   Group: iGEM15_AUC_TURKEY   (2015-09-17)
Revision as of 10:21, 22 September 2015 by Skywalker (Talk | contribs) (→‎Cloning)


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Applications of BBa_K1806005

Cloning

The already linear part was ligated to the pSB1C3 vector, and then transformated to be verified with Colony PCR. The RFP in the iGEM Registry contained Hind3, unlike the RFP in this part. For this reason, the part was cut with the EcoR1 and Hind3 restriction enzymes. The gel runs of the part were examined. The part was tagged as G-Block 7 and sample 7-6 yielded the accurate base pair length.

6005-1.png 6105-2.png

AUC TURKEY 7,6 cutcheck.png 6005-4.png

User Reviews

UNIQ0c06f8f0cf31a777-partinfo-00000000-QINU UNIQ0c06f8f0cf31a777-partinfo-00000001-QINU