Generator
Part:BBa_K1859022:Experience
Designed by: Yusuke Banno Group: iGEM15_Gifu (2015-09-10)
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Applications of BBa_K1859022
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UNIQ10e68156854554e8-partinfo-00000000-QINU UNIQ10e68156854554e8-partinfo-00000001-QINU
We inserted this generator into E. coli K-12 JM109.
We cultivated this bacteria and performed SDS-PAGE without boiling.
Lane G shows this sample.
left: SDS-PAGE before dye / center: SDS-PAGE after dye / right: overlay
There was no polymer RFP at the top of these picture. We found that this generator doesn’t make long chain protein.