RNA

Part:BBa_K1614019:Design

Designed by: Daniel Heid   Group: iGEM15_Heidelberg   (2015-09-18)
Revision as of 14:57, 20 September 2015 by Frieda (Talk | contribs) (→‎Design Notes)

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ATP Aptamer JAWS1 Spinach 2.1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 47
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ATP Aptamer JAWS1 Spinach2.1 is cloned into the RFC 110 and can be transcribed using T7 RNA Polymerase. Cleavage of the HDV is achieved during in vitro transcription. The ATP sensor is a fusion of Spinach2.1 designed by iGEM Team DTU Danemark 2014 and the ATP Aptamer which was improved using the JAWS software. The Aptamer exchanged the second stem loop of Spinach2.1 This enhanced stem increased the fluorescence of the construct.

Source

Synthetic

References