RNA

Part:BBa_K1614012:Design

Designed by: Frieda Anna Sorgenfrei   Group: iGEM15_Heidelberg   (2015-09-16)
Revision as of 14:38, 20 September 2015 by Frieda (Talk | contribs)

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ATP Aptamer Spinach2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 149
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 149
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 69
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 149
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 149
    Illegal NgoMIV site found at 162
    Illegal NgoMIV site found at 191
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110. The ATP Aptamer Spinach2 is generated by fusion of Spinach2 and an ATP Aptamer which exchanged the second stem loop of the Spinach2 construct. By exchanging the loop we achieved a ligand depencency to ATP and therefore achieved just a fluorescence in prencence of DFHBI and ATP.



Source

Synthentic created with JAWS with Spinach II aptamer (Babendure, J.R., Adams, S.R., and Tsien, R.Y. (2003). Aptamers switch on fluorescence of triphenylmethane dyes. Journal of the American Chemical Society 125, 14716-14717.) and ATP aptamer

References