Composite

Part:BBa_K1648003

Designed by: Leung King Pong   Group: iGEM15_Hong_Kong-CUHK   (2015-07-17)
Revision as of 01:41, 19 September 2015 by Fatfaatlai (Talk | contribs)

Construct Map for K1648003

Recombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator

Consist of a template for recombination of mamXY , mamGC and mms operon(K1648001), a promotor & RBS(J13002) in front of each template and a double terminator(B0015) at the back of each template.

This part is designed for homologous recombination to introduce the large magnetosome forming operon into Azotobactor vinelandii.

This part is introduced into A. vinelandii genome via random integration before full operon sequences to be transformed into A. vinelandii.



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1413
    Illegal BamHI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 683
    Illegal NgoMIV site found at 865
    Illegal NgoMIV site found at 955
    Illegal NgoMIV site found at 1363
    Illegal AgeI site found at 566
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 550
    Illegal BsaI site found at 649
    Illegal BsaI site found at 930


[edit]
Categories
Parameters
n/aRecombination Template for mamXY,mamGC and mms Operons with Promotor and Terminator