Part:BBa_K1688018

Nah7 - Upper naphthalene breakdown pathway mutagenized

Upper naphthalene breakdown pathway from the Nah7 plasmid of Pseudomonas putida. The operon consists of ten genes (NahAa, NahAb, NahAc, NahAd, NahB, NahC, NahD, NahE, NahF, NahQ) which in turn codes for seven enzymes that catalyse the breakdown of naphthalene into salicylate. The mutagenized pathway no longer contains the two unwanted PstI restriction sites in the coding sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 4119
  Illegal NotI site found at 8162
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 5212
  Illegal BamHI site found at 3197
  Illegal BamHI site found at 5899
  Illegal XhoI site found at 1308
  Illegal XhoI site found at 3696
  Illegal XhoI site found at 3848
  Illegal XhoI site found at 9350
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1801
  Illegal NgoMIV site found at 3576
  Illegal NgoMIV site found at 5450
  Illegal NgoMIV site found at 6062
  Illegal NgoMIV site found at 9375
  Illegal AgeI site found at 639
  Illegal AgeI site found at 3570
  Illegal AgeI site found at 6278
  Illegal AgeI site found at 8273
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2918
  Illegal BsaI site found at 3591
  Illegal BsaI site found at 4182
  Illegal BsaI site found at 5348
  Illegal BsaI.rc site found at 2752
  Illegal BsaI.rc site found at 5502
  Illegal BsaI.rc site found at 7787
  Illegal SapI.rc site found at 3282
  Illegal SapI.rc site found at 3828
  Illegal SapI.rc site found at 7679