Coding
cadA

Part:BBa_K1602003

Designed by: Alexandra Goretzki, Tanja Habeck, Nina Kuschik-Maczollek, Laurin Monnheimer, Steven Pilger, Bianca Reisinger, Daniel Stumpf, Sebastian Barthel   Group: iGEM15_TU_Darmstadt   (2015-08-28)
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Cis-aconitate decarboxylase (cadA) from Aspergillus terreus

cadA is a gene, coding for a cis-aconitate decarboxylase in Aspergillus terreus. The decarboxylase catalyzes the conversion from cis-aconitate to itaconic acid. The reaction takes place in the cytosol of the host and frees one molecule of carbon dioxide per conversion.
Figure 1 cis-aconitate decarboxylase (coded by cadA)

Characteristics


Molecular Weight 52754.05
Residues 490
Charge -10.0
Isoelectric Point 5.1050
A280 Molar Extinction Coefficients 59360 (reduced) 60110 (cystine bridges)
Improbability of expression in inclusion bodies 0.607
[Data taken from PEPSTATS]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None