Reporter

Part:BBa_K1734006

Designed by: Eduardo Ramírez Montiel   Group: iGEM15_Tec-Monterrey   (2015-09-18)
Revision as of 10:13, 18 September 2015 by Edu92ram (Talk | contribs)

This part encodes a sequence capable to add a purification tag (6X His) at the 3’ terminus of a desired protein. Furthermore, by using the reporter gene (nanoluc) it’s possible to quantify indirectly the protein concentration. The T2A sequence cleaves the protein [1], producing the protein of interest tagged with HisTag and nanoluc separately This part has been codon optimized for Spodoptera frugiperda (Sf9)


Reference: [1] Kim JH, Lee S-R, Li L-H, Park H-J, Park J-H, Lee KY, et al. (2011) High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice. PLoS ONE 6(4): e18556. doi:10.1371/journal.pone.0018556


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 532
[edit]
Categories
Parameters
None