Coding

Part:BBa_K1159001:Design

Designed by: TU Munich 2013   Group: iGEM13_TU-Munich   (2013-05-17)
Revision as of 10:10, 18 September 2015 by Edu92ram (Talk | contribs) (→‎Design Notes)

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NanoLuc Luciferase in RFC[25]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Keywords:


Abbreviations:

Design Notes

Related BioBrick: Other versions:BBa_K1734004: Nanoluc (Codon optimized for Sf9 cells)

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:



Parameters for BioBrick BBa_K1159001 automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 10: (underlined part encodes the protein)
 ATGGCCGGC ... GCTACCGGTTAA
 ORF from 1 to 525 (excluding stop-codon)
Amino acid sequence:
  1 MAGVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGT
101 LVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRLCERILATG
Amino acid composition:
A (Ala)4 (2.3%)
R (Arg)7 (4.0%)
N (Asn)8 (4.6%)
D (Asp)12 (6.9%)
C (Cys)1 (0.6%)
Q (Gln)7 (4.0%)
E (Glu)8 (4.6%)
G (Gly)22 (12.6%)
H (His)4 (2.3%)
I (Ile)18 (10.3%)
L (Leu)16 (9.1%)
K (Lys)7 (4.0%)
M (Met)3 (1.7%)
F (Phe)8 (4.6%)
P (Pro)6 (3.4%)
S (Ser)6 (3.4%)
T (Thr)11 (6.3%)
W (Trp)3 (1.7%)
Y (Tyr)6 (3.4%)
V (Val)18 (10.3%)
Amino acid counting:  
Total number:175
Positively charged (Arg+Lys):14 (8.0%)
Negatively charged (Asp+Glu):20 (11.4%)
Biochemical parameters:
Molecular mass [Da]:19381.3
Theoretical pI:5.10
Extinction coefficient at 280 nm [M-1 cm-1]:25440 / 25503 (all Cys red/ox)
Organism specific parameters
Organism
E. coli
B. subtilis
S. cervisiae
A. thaliana
Mammals
Estimated half-life [h]
>10
Platzhalter
>20
Platzhalter
30
Codon usage (CAI)
good (0.66)
B. subtilis
good (0.62)
A. thaliana
excellent (0.82)
Organism specific parameters
Organism:E. coliB. subtilisS. cervisiaeA. thalianaMammals
Estimated half-life [h]>10Platzhalter>20Platzhalter30
Codon usage (CAI)good (0.66)B. subtilisgood (0.62)A. thalianaexcellent (0.82)
Sequence features:
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Source

Source:


Organism:


References

Literature references:

Database references: