Part:BBa_K1732010
pSB1C3-J23100-B0034-E0040-His-B0015
pSB1C3-J23100-B0034-E0040-His-B0015
His tagged E0040 GFP and strong RBS. The J23100 strong constitutive promoter allows for high level expression of the fluorescent protein used to measure fluorescence.
A set of fluorescent proteins which includes BFP, GFP, eGFP, E0040 GFP, OFP, YFP, and RFP with a J23100 constitutive promoter and RFP with a J23115 constitutive promoter were characterized. The relative fluorescence as well as the signal-to-noise ratio of all the proteins in the MACH competent cell line were calculated. eGFP was measured at (ex/em = 501nm/511nm).
Sequence alignment of original sequence to codon optimized sequence, indicating the differences in amino acids.
The graphs below show the data for the original set of fluorescent proteins prior to being codon optimized via the IDT codon optimization tool. The set consists of His tagged RFP, OFP, YFP, eGFP, and BFP, each with a strong RBS and WT lac promoter that allows for Lac induction of fluorescence.
[[File:Fluorescent_proteins_characterization.jpg[]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 212
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 77
| None |