Coding
ccdB-rev

Part:BBa_K1616012

Designed by: Johanna Chesnel   Group: iGEM15_IONIS_Paris   (2015-09-18)
Revision as of 08:38, 18 September 2015 by Chesne j (Talk | contribs)

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ccdB reversed


ccdB comes from the ccd system in Escherichia coli F plasmid and acts as a gyrase poison.


Usage and Biology

This part is the reversed sequence of ccdB and can be used with a reversed promoter, reversed RBS and reversed terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 89


Design Notes

Illegal sites have been checked.


Source

This part is the reversed sequence of the ccdB part: BBa_P1016.

References

[edit]
Categories
Parameters
None