Regulatory

Part:BBa_K1840002:Design

Designed by: Julia Anna Adrian   Group: iGEM15_NTNU_Trondheim   (2015-09-18)
Revision as of 07:54, 18 September 2015 by Julia.A Adrian (Talk | contribs) (→‎References)

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Zwf promotor from Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The considerations we made regarded the starting and endpoint of the promotor. We only know that it has to be in front of the zwf gene. We assumed it starts directly after the previous gene. This gene codes for the repressor, HexR, and is situated on the opposite strand.


Source

The part was designed after information from the paper by DADDAOUA, A., KRELL, T. & RAMOS, J.-L. 2009. Regulation of Glucose Metabolism in Pseudomonas: The Phosphorylative Branch and Entner-Doudoroff Enzymes are Regulated by a Repressor Containing a Sugar Iisomerase Domain. Journal of Biological Chemistry, 284, 21360-21368.

References

DADDAOUA, A., KRELL, T. & RAMOS, J.-L. 2009. Regulation of Glucose Metabolism in Pseudomonas: The Phosphorylative Branch and Entner-Doudoroff Enzymes are Regulated by a Repressor Containing a Sugar Iisomerase Domain. Journal of Biological Chemistry, 284, 21360-21368