Part:BBa_K1701002

PcotC

The PcotC gene is the promoter of the CotC gene, and the CotC gene codes for the protein CotC.The CotC protein, in Bacillus subtilis, is one of the the protein component of its spore coat, such as Cot B, Cot C, Cot F, and can be used as a fusion partner to express proteins or polypeptides on the spore surface.

We get the sequence of PcotC from the whole genome shotgun sequence of Bacillus subtilis subsp. subtilis str. NCIB 3610 chromosome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Results

B. subtilis cells carrying fusion proteins (CotC-EGFP) were fixed for visualization under a fluorescence microscope. The same batch of B. subtilis cells without fusion proteins was used as s control. Strong fluorescence was observed when the B. subtilis cells expressed the fusion proteins (CotC-EGFP), whereas no fluorescence was detected for the control group. Taken together, these results confirmed the fusion proteins can express well on the surface of B. subtilis. The following pictures show the results of fluorescence measurements.

Figure 3. Fluorescence measurement of B. subtilis cells containing the fusion proteins. b) Fluorescence intensity of the fusion proteins Pveg-CotC-EGFP and Pcot-CotC-EGFP. c) A visible photograph of b).