Composite

Part:BBa_K1696011

Designed by: Yue Liu   Group: iGEM15_TJU   (2015-09-14)
Revision as of 06:51, 17 September 2015 by Ggjyliuyue (Talk | contribs)

Riboflavin producing parts

This part is based on the BBa_K1172303 in Part Registry constructed by 2013 Team Bielefelf-Germany which was also aimed at producing riboflavins.

The riboflavin biosynthesis genes ribABDEC from E. coli are overexpressed under the control of the inducible trc promoter. Enzymes encoded by the genes shown are: ribA, GTP cyclohydrolase II; ribB, 3,4-dihydroxy-2-butanone 4-phosphate synthase; ribD, fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/ 5-amino-6-(5-phosphoribosylamino)uracil reductase; ribE, 6,7-dimethyl-8-ribityllumazine synthase; ribC, riboflavin synthase gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3410
    Illegal BamHI site found at 1370
    Illegal XhoI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 262
    Illegal AgeI site found at 992
    Illegal AgeI site found at 1034
    Illegal AgeI site found at 2752
    Illegal AgeI site found at 3163
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None